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Invitrogen™ LR Clonase™ II Plus enzyme. Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes. Manual de uso de la tecnología de clonacióon Multisite Gateway. multisite pro using technology LR Clonase™ II Plus Enzyme Mix 20 reactions 100 reactionsGateway LR Clonase enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the Gateway® LR Clonase™ II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination proteins. pDEST™ R4-R3 Vector II (supercoiled, 20 fmol). 1 μl. 1x TE Buffer, pH 8.0 to 8 μl. 2. Vortex LR Clonase® II Plus enzyme mix briefly. 2. Vortex LR Clonase™ II enzyme mix briefly. Add 2 μl to the components above This manual provides an overview of the Gateway® Technology and provides.
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