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SUMMARY AND EXPLANATION. Previous tests for the detection of indole production required a xylene extraction procedure to detect small amounts of indole Upon completion of this exercise, a student should be able to: • Understand the underlying mechanism of the indole test;. • Properly conduct the indole test;The indole test determines the ability of an organism to produce indole from the breakdown of the amino acid tryptophan. Tryptophan is hydrolyzed by
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