Autoclave sterilization pdf

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Various pieces of equipment are used for sterilization in laboratory animal facilities, but the autoclave is the primary means of sterilizing supplies. 2. Some equipment must be supplied sterile and discarded following use. Methods Approved for Sterilization: • Autoclaves that use heat and steam under pressure. sterilization by autoclaving. ASSOCIATED RISKS: Autoclaves are sterilizers using high pressure and high temperature steam. The potential. Steam sterilization generally denotes heating in an autoclave utilizing saturated steam under a pressure of approximately 15 pounds per square inch (psi) to This SOP also applies to the sterilization by autoclaving of different materials and items that cdc.gov/biosafety/publications/bmbl5/BMBL.pdf. STERILIZATION GUIDELINES. 9.8. Autoclave with vacuum pump. 24. 9.8.1. Checking the airtight seal before sterilization = Bowie‑Dick test. It can be achieved by high-pressure steam (autoclave), dry heat (oven), chemical sterilants (glutaraldehydes or formaldehyde solutions) or physical agents (The sterilization of materials using steam and pressure is a dependable procedure for the destruction of all forms of microbial life. However, the autoclave